NG5A CryoPreserv

Next Generation Cryopreservation Solution for Primary Cells

NG5A CryoPreserv is a propriety biopreservation medium designed for the long-term storage and transport of primary cells at ultra-low temperatures (–70°C to –196°C). The formulation is specifically engineered to reduce cellular stress responses during both cryopreservation and thawing, thereby minimizing apoptosis and maintaining post-thaw cell viability and functional integrity. Formulated without serum, proteins, antibiotics, hormones, or growth factors, NG5A CryoPreserv is chemically defined, animal-free, and eliminates the risks of contamination, ensuring reproducible and reliable results across various applications.

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Ready-to-Use: Simplifies workflows and reduces preparation time.
cGMP Grade: Manufactured in accordance with current Good Manufacturing Practices to meet the highest standards.
Chemically Defined: Guarantees consistent performance across batches and reduces the risk of cross-contamination.
Endotoxin & Sterility Tested: Assures safety and purity for use in clinical and commercial applications.
Low Variability: Enhanced reproducibility with lot-to-lot consistency.
FDA Registered Facility: Produced in a facility registered with the FDA, ensuring compliance with regulatory standards.
Animal-Free / Serum-Free: Ideal for ensuring ethical standards and eliminating variability introduced by animal-derived components.

ColdPreserv Medium
Catalog Number: CQ-HPS-500; Volume: 500 mL

ColdPreserv Medium is shipped at a cold temperature. Store at 2°-8°C away from direct light. ColdPreserv Medium is for research use or for further manufacturing. To order this item, please contact us at info@vitroprep.com.

ColdPreserv Medium Usage and Hypothermic Preservation Protocol:

ColdPreserv Medium is a ready-to-use solution. Do not add any additives to this medium.
Following the isolation and clean-up of the primary cells, place the cells into 0°C to 4°C ColdPreserv Medium at a density of 1-3 × 106 cells/mL. Place the cells into a 4°C refrigerator or onto wet ice for storage.
It is recommended to allow the cells to recover in ColdPreserv Medium for at least 60 minutes prior to counting or freezing. The cold incubation allows time for the cells to repair damage incurred during the isolation process and can result in an increase in viability.
The cells should be placed into a flat-bottom vessel (not conical) during storage to prevent the cells from piling onto one another.
If freezing, the cells should be spun out of ColdPreserv Medium and frozen within 24 hours.