Liver Perfusion Solution I is a proprietary, chemically defined chelating buffer formulated to gently disrupt intercellular junctions between hepatocytes, facilitating efficient tissue dissociation and generation of high-quality single-cell suspensions. Free from serum, proteins, antibiotics, hormones, and growth factors, this animal-origin–free solution ensures consistency, reproducibility, and regulatory compliance for downstream applications in hepatocyte isolation and liver cell biology research.
Request a QuoteLiver Perfusion Solution I is shipped at cold temperature. Store at 2°C-8°C, away from direct light. Liver Perfusion Solution I is for research use or further manufacturing. To order this item, please contact us at info@vitroprep.com.
Tissue should be collected from the donor with as much intact vasculature as possible, specifically the portal vein. Perfusion of the tissue can take place through any of the major vessels (superior vena cava, inferior vena cava, or portal vein); however, the portal vein is recommended due to its capacity to reach 80-100% of the liver mass. If perfusing through the portal vein, the SVC should be completely closed off, leaving the IVC open or restricted to allow the perfusate to exit. The perfusion rate should be high enough to achieve adequate tissue inflation (chelating buffer infiltration) without overly inflating the tissue and introducing too much pressure on the cells. A rate of 0.4-0.6 mL/gram of tissue/minute is recommended. However, constant observation of the tissue throughout the perfusion process should still be done to avoid under- or over-inflation. Depending on the amount of residual blood remaining when the tissue was received, the chelation perfusion should take 10-14 minutes.
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