CytoBase Solution

VitroPrep™ Cytology Processing Kit

The VitroPrep™ Cytology Processing Kit delivers high-quality specimen preparation for clear, unobstructed cellular analysis. As a second-generation liquid-based cytology (LBC) solution, it offers a streamlined, manual workflow—eliminating the need for automation.
The kit effectively preserves cellular integrity, lyses red blood cells, removes debris, and produces a uniform monolayer of cells on standard microscope slides. All formulations are developed using advanced Green Chemistry principles, ensuring safety and sustainability without compromising performance.

VitroPrep™ includes two proprietary solutions: PreservPlus™ Solution and CytoBase™ Solution, engineered to maximize specimen quality and diagnostic reliability.

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  • CytoBase™ Solution is a proprietary formulation designed to facilitate robust monolayer cell adhesion on standard microscopic glass slides.
  • The solution is compatible with a wide range of standard histological stains, making it a versatile choice for various research applications.
  • No post-treatment heating is required after application of CytoBase™ Solution, ensuring ease of use and consistent results.
  • Formulated using sustainable Green Chemistry principles, CytoBase™ Solution offers an environmentally responsible option without compromising performance.

CytoBase Solution
Catalog Number: CQ-0012C

  1. CytoBase™ Solution is a proprietary formulation designed to facilitate robust monolayer cell adhesion on standard microscopic glass slides.
  2. No post-treatment heating is required after application of CytoBase™ Solution, ensuring ease of use and consistent results.
  3. The solution is compatible with a wide range of standard histological stains, making it a versatile choice for various research applications.
  4. Formulated using sustainable Green Chemistry principles, CytoBase™ Solution offers an environmentally responsible option without compromising performance.
  5. 30 pre-filled 12 mL vials of CytoBase™ Solution per box for efficient, ready-to-use applications.
  6. Store at room temperature (20–30°C), protect from direct light.

VitroPrepTM Sample Processing Procedure

  1. Vortex PreservPlusTM Solution containing specimen* for about 10 seconds.
  2. Pour the specimen into a 15 mL conical centrifuge tube.
  3. Centrifuge the tube for 10 min @ 1000 ×g.
  4. Gently and quickly decant the supernatant liquid from the tube.
  5. Depending on the cellular pellet size, add CytoBaseTM Solution in a ratio of 3 parts CytoBaseTM Solution to 1 part of cellular pellet, typically 250-300 μL, into the tube and vortex the tube about 20 seconds.
  6. Withdraw 45 µL of the mixture (step 5) and spread gently on a standard glass slide.
  7. Allow drying of the slide at room temperature for 3-4 hours, and follow standard Papanicolau staining protocol.

CytoBase Solution Safety Data Sheet

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